05-581
Anti-CFTR Antibody, clone MM13-4
clone MM13-4, Upstate®, from mouse
Synonym(s):
ATP-binding cassette sub-family C, member 7, ATP-binding cassette transporter sub-family C member 7, cAMP-dependent chloride channel, cystic fibrosis transmembrane conductance regulator, cystic fibrosis transmembrane conductance regulator (ATP-binding ca
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
MM13-4, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CFTR(1080)
General description
CFTR (Cystic Fibrosis Transmembrane conductance Regulator) is a 170 kDa chloride (Cl-) channel that regulates salt and water transport across epithelial tissues, where it modulates production of mucus, sweat, saliva, tears, and digestive enzymes. Cystic fibrosis patients have mutations in this protein, resulting in defective in ion transport. Symptoms include reduced transport of sodium chloride (saltier sweat) and digestive and respiratory disorders caused by thicker mucus. CFTR mutations are also associated with congenital bilateral absence of the vas deferens in males.
Specificity
Other species cross-reactivity not tested.
This antibody recognizes CFTR, Mr 170kDa. Two additional proteins were detected, Mr 52 and 38 kDa, in CFTR-transfected BHK cell lysates.
Immunogen
Synthetic peptide (RKGYRQRLELSD) corresponding to residues 25-36 of human cystic fibrosis transmembrane conductance regulator (CFTR). The immunizing sequence is identical in monkey and rabbit, has 11/12 identical amino acids in sheep, cow and frog, 10/12 identity in mouse and 9/12 identity in rat. Clone MM13-4.
Application
Anti-CFTR Antibody, clone MM13-4 detects level of CFTR & has been published & validated for use in IC, IP & WB.
Immunoprecipitation:
This antibody has been shown to immunoprecipitate CFTR from a T84 cell lysate by an independent laboratory.
Immunocytochemistry:
This antibody has been reported to immunostain CFTR in T84 cells.
This antibody has been shown to immunoprecipitate CFTR from a T84 cell lysate by an independent laboratory.
Immunocytochemistry:
This antibody has been reported to immunostain CFTR in T84 cells.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Quality
Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or CFTR-transfected BHK.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected CFTR in human T84 colon carcinoma epithelial RIPA cell lysates. A previous lot detected CFTR in CFTR-transfected BHK.
Note: CFTR can run as a diffuse protein on SDS-PAGE.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected CFTR in human T84 colon carcinoma epithelial RIPA cell lysates. A previous lot detected CFTR in CFTR-transfected BHK.
Note: CFTR can run as a diffuse protein on SDS-PAGE.
Target description
170 kDa
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1k in buffer containing 0.02M phosphate buffer, pH 7.6, 0.25M NaCl, and 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Pancreas. RIPA cell lysates
Pancreas. RIPA cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Analysis of CFTR folding and degradation in transiently transfected cells.
Diane E Grove,Meredith F N Rosser,Richard L Watkins,Douglas M Cyr
Methods in Molecular Biology null
Irene Cantone et al.
Nature communications, 7, 12354-12354 (2016-08-11)
Erasure of epigenetic memory is required to convert somatic cells towards pluripotency. Reactivation of the inactive X chromosome (Xi) has been used to model epigenetic reprogramming in mouse, but human studies are hampered by Xi epigenetic instability and difficulties in
Onofrio Laselva et al.
iScience, 24(6), 102542-102542 (2021-06-19)
Ivacaftor (VX-770) was the first cystic fibrosis transmembrane conductance regulator (CFTR) modulatory drug approved for the treatment of patients with cystic fibrosis. Electron cryomicroscopy (cryo-EM) studies of detergent-solubilized CFTR indicated that VX-770 bound to a site at the interface between
J Michael Younger et al.
Methods in molecular biology (Clifton, N.J.), 301, 293-303 (2005-05-27)
Components of the ubiquitin-proteasome system function on the surface of the endoplasmic reticulum (ER) to select misfolded proteins for degradation. Herein we describe methods that allow for the study of the pathway for proteasomal degradation of the cystic fibrosis transmembrane
Do Yeon Cho et al.
Redox biology, 43, 101998-101998 (2021-05-11)
Lipopolysaccharide (LPS) serves as the interface between gram-negative bacteria (GNB) and the innate immune response in respiratory epithelial cells (REC). Herein, we describe a novel biological role of LPS that permits GNB to persist in the respiratory tract through inducing
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