HPLC Analysis of Active Cannabinoid Compounds in Gummy Bear Candy on Ascentis^® Express Biphenyl with UV Detection after Extraction

extraction tube

SupelQue Non-buffered tube 2 (55295-U)

extraction process

Transfer the solution into 50 mL plastic tube (55248-U); add 10 mL acetonitrile; shake for 1 minute by hand; add contents of the Supel QuE Non-Buffered Extraction Tube 2 (55295-U); (shake for 5 minutes using QuEChERS shaker (55278-U); centrifuge for 5 minutes at 5000 rpm; collect top acetonitrile layer; no cleanup is required.)

column

Ascentis Express Biphenyl, 10cm x 2.1mm, 2.7 μm particles (64065-U)

mobile phase

(A) 0.1% TFA in water; (B) 0.1% TFA in acetonitrile

gradient

47% B to 50% B in 13 min.; 100% B in 0.1 min; held at 100% B for 3 min; 47% B in 0.1 min; held at 47% B for 2.5 min.

flow rate

0.7 mL/min

pressure

5000 psi (345 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

5 μL