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LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5

LC/MS/MS Analysis of Epinephrine, Metanephrine and Metabolites on Ascentis® Express OH5 application for HPLC

Materiales

Analytical column

Referencia del producto
Descripción
Precios

53757-U

Ascentis® Express OH5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Referencia del producto
Descripción
Precios

C-109

Catecholamine Mix 1 (Epinephrines) solution

1.0 mg/mL each component in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

C-110

Catecholamine Mix 2 (Metanephrines) solution

1.0 mg/mL each component in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)

mobile phase

[A] 5 mM ammonium formate; [B] 5 mM ammonium  formate in acetonitrile; (15:85, A:B)

flow rate

0.3 mL/min

pressure

1189 psi (82 bar)

column temp.

35 °C

detector

MS ESI(+), 100-1000 m/z

injection

1 μL

sample

300 ng/mL each in acetonitrile

Descripción

Analysis Note

HILIC mode on Ascentis Express OH5 provided rapid separation of neurotransmitters in highly MS-compatible mobile phases. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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