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LC/MS Analysis of Melamine and Hydrolysis Products on Ascentis® Express HILIC

LC/MS Analysis of Melamine and Hydrolysis Products on Ascentis® Express HILIC application for HPLC

Materiales

Analytical column

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Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

mobile phase component

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Formato de amonio

eluent additive for LC-MS, LiChropur, ≥99.0%

standard

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Melamine

99%

Ammelide

analytical standard

CONDITIONS

column

Ascentis Express HILIC, 5 cm x 2.1 mm I.D., 2.7 μm particles (53934-U)

mobile phase

5 mM ammonium formate in 95:5 (v/v) acetonitrile:water

flow rate

0.2 mL/min

column temp.

35 °C

detector

MS, ESI(+), full scan

injection

2 μL

sample

1 mg/L in mobile phase

Descripción

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany