HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery^® HS C18 (Acetone-Acetonitrile Mobile Phase, ELSD Detection)

column

Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)

mobile phase

[A] acetone; [B] acetonitrile; (60:40, A:B)

flow rate

1.5 mL/min

pressure

914 psi (63 bar)

column temp.

30 °C

detector

ELSD

injection

5 μL

sample

5% soybean oil in acetone (w/v)