HPLC Analysis of Recombinant Tumor Necrosis Factor Receptor-Fc Fusion Protein (rhTNFR-Fc) on Zenix^® SEC-300 versus SRT^® SEC-300, Effect of Particle Size on Resolution

column

Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777051)

column

SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm, 300 Å particles (Z777051) (Z777033)

mobile phase

150 mM sodium phosphate, pH 7.0

flow rate

1 mL/min

detector

UV, 214 nm

injection

20 μL

sample

Recombinant tumor necrosis factor receptor-Fc fusion protein (rhTNFR-Fc), including oligomers (440 kD and 360 kD), monomer (150 kD), and impurity (120 kD)