HPLC Analysis of Phenolics from Rosemary on Ascentis^® Express C8

column

Ascentis Express C8, 5 cm x 3 mm I.D., 2.7 μm particles (53848-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile

gradient

15 to 100% B in 8.5 min

flow rate

0.6 mL/min

column temp.

35 °C

detector

UV, 214 nm

injection

2 μL

sample

100 mg/L in 75 μM EDTA, 1.5 mM ascorbate, 15% acetonitrile