HPLC Analysis of Luciferin Enantiomers on Astec^® CHIROBIOTIC^® V

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] 20 mM ammonium nitrate, pH 5.5; [B] THF; (90:10, A:B)

flow rate

1 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

6 μL

sample

0.5 mg/mL in methanol