HPLC Analysis of Ketoprofen Enantiomers on Astec^® CHIROBIOTIC^® R

column

CHIROBIOTIC R, 15 cm x 4.6 mm I.D., 5 μm particles (13023AST)

mobile phase

[A] 20 mM ammonium acetate, pH 5.6; [B] methanol; (50:50, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

5 μL

sample

1 mg/mL in methanol