HPLC Analysis of Biotin Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] 0.1% sodium phosphate, pH 4.1; [B] methanol; (60:40, A:B)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 210 nm

injection

2 μL

sample

4 mg/mL in methanol