HPLC Analysis of Aflatoxins on Ascentis^® Express C18 with Fluorescence Detection

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] water; [B] acetonitrile; (75:25, A:B)

flow rate

2.0 mL/min

pressure

6381 psi (440 bar)

column temp.

35 °C

detector

fluorescence, ex 360 nm, em 440 nm

injection

50 μL

sample

200 μL aflatoxin standard in acetonitrile; 0.5 μg/mL B2 and G2; 2 μg/mL B1 and G1; 800 μL delivatization solution: 70% water, 20% TFA and 10% acetic acid
heat for 9 min at 65 °C