HPLC Analysis of 13 Aldehyde/Ketone DNPH Derivatives on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 μm particles (53827-U)

mobile phase

[A] water; [B] acetonitrile; (40:60 v/v, A:B)

flow rate

2 mL/min

pressure

4685 psi (323 bar)

detector

UV, 360 nm

injection

10 μL

sample

carb Method 1004 DNPH Mix 2 (47651-U) dilute 3:2 with water