Saltar al contenido
Merck

Photocrosslinking of 5-iodouracil-substituted RNA and DNA to proteins.

Science (New York, N.Y.) (1993-11-19)
M C Willis, B J Hicke, O C Uhlenbeck, T R Cech, T H Koch
RESUMEN

5-Iodouracil-substituted RNA and DNA were crosslinked regiospecifically to associated proteins in yields of 70 to 94% of bound nucleic acid. Irradiation of the iodouracil chromophore with monochromatic, long-wavelength ultraviolet radiation (325 nanometers) eliminates excitation of other nucleic acid and protein chromophores. The combination of high crosslinking yields, excellent specificity, and elimination of photodamage to other chromophores represents an important advance toward the precise identification of contacts in nucleoprotein complexes.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
5-Iodouracil, 98%