Saltar al contenido
Merck
  • A Protocol for the Multi-Omic Integration of Cervical Microbiota and Urine Metabolomics to Understand Human Papillomavirus (HPV)-Driven Dysbiosis.

A Protocol for the Multi-Omic Integration of Cervical Microbiota and Urine Metabolomics to Understand Human Papillomavirus (HPV)-Driven Dysbiosis.

Biomedicines (2020-04-12)
Nataliya Chorna, Filipa Godoy-Vitorino
RESUMEN

The multi-omic integration of microbiota data with metabolomics has gained popularity. This protocol is based on a human multi-omics study, integrating cervicovaginal microbiota, HPV status and neoplasia, with urinary metabolites. Indeed, to understand the biology of the infections and to develop adequate interventions for cervical cancer prevention, studies are needed to characterize in detail the cervical microbiota and understand the systemic metabolome. This article is a detailed protocol for the multi-omic integration of cervical microbiota and urine metabolome to shed light on the systemic effects of cervical dysbioses associated with Human Papillomavirus (HPV) infections. This methods article suggests detailed sample collection and laboratory processes of metabolomics, DNA extraction for microbiota, HPV typing, and the bioinformatic analyses of the data, both to characterize the metabolome, the microbiota, and joint multi-omic analyses, useful for the development of new point-of-care diagnostic tests based on these approaches.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Supelco
Certified glass inserts for 8 x 40 mm shell vials, volume 100 μL, clear glass insert (with plastic bottom spring), O.D. × H 5 mm × 30 mm, pkg of 100 ea
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Supelco
Assembled Screw Cap with hole and PTFE/silicone septum, black phenolic hole cap, thread for 8-425, PTFE/silicone, septum thickness 1.5 mm, for use with 2 mL screw thead vial, 4.6 mm opening, pkg of 100 ea
En este momento no podemos mostrarle ni los precios ni la disponibilidad