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Key Documents

ZRB2630

Sigma-Aldrich

Anti-Rab14 Antibody, clone 1M15 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Sinónimos:

Ras-related protein Rab-14

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

recombinant monoclonal

description

Clone, 1M15

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 24 kDa
observed mol wt ~24 kDa

purified by

using Protein A

species reactivity

mouse, human, rat

packaging

antibody small pack of 25

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... RAB14(51552)

Categorías relacionadas

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1M15 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Ras-related protein Rab-14. It targets an epitope within 23 amino acids from the C-terminal region.

Immunogen

KLH-conjugated liner peptide corresponding to 23 amino acids from the C-terminal region of human Ras-related protein Rab-14.

Application

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Rab14 in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Rab14 in lysate from Mouse brain tissue and 1:1,000 dilution detected Rab14 in Rat brain tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Rab14 in Human testis tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Rab14 peptide with a KD of 8.4 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rab14 in U-251 MG cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Ras-related protein Rab-14 (UniProt: P61106; also known as Rab14) is encoded by the RAB14 gene (Gene ID: 51552) in human. Rab proteins constitute a large family of the low-molecular-weight GTPases that play key roles in the regulation of intracellular membrane trafficking. They also play a role in cargo selection, vesicle budding, vesicle motility, tethering, docking, and fusion. They act as molecular switches that flip between a GDP-bound inactive state and a GTP-bound active form in which they recruit downstream effector proteins onto membranes. Rab14 is involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. It is reported to regulate the Golgi to endosome transport of FGFR-containing vesicles during early development. It may also act by modulating the kinesin KIF16B-cargo association to endosomes. It is also involved in phagosome and early endosome fusion. It is shown to be essential for the formation of PtdIns(4,5)P2-enriched lipid domains that promote the recruitment of downstream effectors of polarity, including Par3, aPKC and Cdc42. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Linford, A., et al. (2012). Dev. Cell. 22(5); 952-966; Junutula, JR., et al. (2004). Mol. Biol. Cell. 15(5); 2218-2229).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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