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U1500

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type III, powder, 15,000-50,000 units/g solid

Sinónimos:

Jack bean urease, Urea amidohydrolase

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About This Item

Número de CAS:
9002-13-5
Comisión internacional de enzimas:
3.5.1.5 (BRENDA, IUBMB)
EC Number:
232-656-0
MDL number:
MFCD00070858
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Canavalia ensiformis

Quality Level

300

type

Type III

form

powder

specific activity

15,000-50,000 units/g solid

mol wt

~544620 Da

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.

Biochem/physiol Actions

Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.

Unit Definition

1 U setzt 1.0 μmol NH3 aus Harnstoff pro Minute bei pH 7.0 und 25 °C frei. Es ist äquivalent zu 1.0 I.U. oder 0.054 Sumner Einheiten (1.0 mg Ammoniak Stickstoff in 5 Minuten bei pH 7.0 und 20 °C)

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Han-Ho Kim et al.
Journal of microbiology and biotechnology, 31(12), 1684-1691 (2021-12-29)
In this study, the physicochemical changes related to fishy smell were determined by storing high hydrostatic pressure (HHP)-treated mackerel (Scomber japonicus) meat in a refrigerator for 20 days. The inhibition of crude urease activity from Vibrio parahaemolyticus using HHP treatment
Karl Norris et al.
Marine drugs, 18(6) (2020-06-06)
Whey protein isolate (WPI) is a by-product from the production of cheese and Greek yoghurt comprising β-lactoglobulin (β-lg) (75%). Hydrogels can be produced from WPI solutions through heating; hydrogels can be sterilized by autoclaving. WPI hydrogels have shown cytocompatibility and
Jungyeon Kim et al.
PloS one, 15(3), e0230072-e0230072 (2020-03-18)
In the urinary metabolomics for finding biomarkers in urine, owing to high concentrations of urea, for chromatography-based metabolomic analysis, urea needed to be degraded by urease. This urease pretreatment has been the key step of sample preparation for standard urinary
Sun-Il Kim et al.
Microorganisms, 10(1) (2022-01-22)
Ammonia from livestock manure reacts with chemical components discharged from various emission sources to produce airborne particulate matter. This study aimed to investigate a novel effective microbial agent to suppress ammonia gas emitted from manure. Both isolated L12I and 12III
Fernanda Stanisçuaski et al.
Journal of insect physiology, 55(3), 255-263 (2009-01-06)
Ureases are metalloenzymes that are widespread among plants, fungi and bacteria. Urease isoforms (jack bean urease-JBU and canatoxin) from Canavalia ensiformis seeds are toxic to insects and fungi, suggesting a role in plant defense. The entomotoxic effect is due to
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