T7660

Tetracycline hydrochloride

powder, BioReagent, suitable for cell culture

• Sinónimos: Tetracycline HCL
• Fórmula empírica (notación de Hill): C₂₂H₂₄N₂O₈ · HCl
• Número de CAS: 64-75-5
• Peso molecular: 480.90
• Beilstein: 3844873
• Número CE: 200-593-8
• Número MDL: MFCD00078142
• Código UNSPSC: 51284043
• ID de la sustancia en PubChem: 24900468
• NACRES: NA.76

Propiedades

• Línea del producto: BioReagent
• Nivel de calidad: 200
• Formulario: powder
• condiciones de almacenamiento: (Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• color: yellow
• mp: 220-223 °C (lit.)
• espectro de actividad antibiótica: Gram-negative bacteria; Gram-positive bacteria
• Modo de acción: protein synthesis | interferes
• temp. de almacenamiento: −20°C
• cadena SMILES: Cl.CN(C)[C@H]1[C@@H]2C[C@H]3C(=C(O)[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c4c(O)cccc4[C@@]3(C)O
• InChI: 1S/C22H24N2O8.ClH/c1-21(31)8-5-4-6-11(25)12(8)16(26)13-9(21)7-10-15(24(2)3)17(27)14(20(23)30)19(29)22(10,32)18(13)28;/h4-6,9-10,15,25,27-28,31-32H,7H2,1-3H3,(H2,23,30);1H/t9-,10-,15-,21+,22-;/m0./s1
• Clave InChI: XMEVHPAGJVLHIG-FMZCEJRJSA-N

Descripción

Descripción general

Chemical structure: tetracycline

Aplicación

Tetracycline is a broad spectrum polyketide antibiotic with clinical uses in treating bacterial infections such as Rocky Mountain spotted fever, typush fever, tick fevers, Q fever, and Brill-Zinsser disease and to treat upper respiratory infections and acne. It has been used in studies of multidrug resistance and potential side effects including acute pancreatitis. It is recommended for use in cell culture applications at 10 mg/L.

Acciones bioquímicas o fisiológicas

Mode of Action: Tetracycline passively diffuses through proin channels in the cell membrane, binding to 30S ribosomes and inhibits protein synthesis by preventing access of aminoacyl tRNA to the acceptor site on the mRNA-ribosome complex. It also binds to the bacterial 50S ribosomal subunit, altering the membrane and causing intracellular components to leak from bacterial cells. The inhibitory effects can be reversed by washing, suggesting that it is the reversibly bound antibiotic, and not the irreversibly bound drug, that is responsible for antibacterial action.

Mode of Resistance: The effects are inactivated via a loss of cell wall permeability.

Antimicrobial spectrum: Includes a wide range of antimicrobial activity against gram-positive and gram-negative bacteria.

Precaución

This product should be frozen below 0°C and protected from light and moisture. In these conditions, the product has been shown to retain activity for 4 years. Stock solutions should be stored at -20°C and are stable at 37°C for 4 days.

Nota de preparación

The product is freely soluble in water, slightly soluble in 96% ethanol, and is practically insoluble in acetone. Tetracycline is rapidly destroyed by alkali hydroxide solutions and standing water solutions become turbid due to hydrolysis and precipitation. The potency of tetracycline is reduced in solutions with pH below 2.

Otras notas

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place

Información de seguridad

• Pictogramas: GHS08,GHS07,GHS09
• Palabra de señalización: Warning
• Frases de peligro: H315,H319,H335,H361fd,H410
• Consejos de prudencia: P202 - P261 - P273 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Clasificaciones de peligro: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves