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Merck

T6140

Sigma-Aldrich

N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt

plasmin substrate, chromogenic, ≥98% (TLC), powder

Sinónimos:

N-Tosylglycyl-L-prolyl-L-lysine 4-nitroanilide acetate salt

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About This Item

Fórmula lineal:
C26H34N6O7S · C2H4O2
Número de CAS:
Peso molecular:
634.70
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

product name

N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt, plasmin substrate

Quality Level

assay

≥98% (TLC)

form

powder

solubility

ethanol: 50 mg/mL, colorless to yellow

storage temp.

−20°C

SMILES string

CC(O)=O.Cc1ccc(cc1)S(=O)(=O)NCC(=O)N2CCC[C@H]2C(=O)N[C@@H](CCCCN)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C26H34N6O7S.C2H4O2/c1-18-7-13-21(14-8-18)40(38,39)28-17-24(33)31-16-4-6-23(31)26(35)30-22(5-2-3-15-27)25(34)29-19-9-11-20(12-10-19)32(36)37;1-2(3)4/h7-14,22-23,28H,2-6,15-17,27H2,1H3,(H,29,34)(H,30,35);1H3,(H,3,4)/t22-,23-;/m0./s1

InChI key

KPGSNEYSJPIZCW-SJEIDVEUSA-N

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Application

N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt has been used:
  • as a substrate for examining the amidolytic activity of aprE2, aprE5-41
  • as a substrate for serine protease and fibrinogenolytic assays
  • as a substrate for plasmin assay

Biochem/physiol Actions

N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt is a chromogenic substrate for plasmin assay, serine protease enzyme activity and fibrinogenolytic assays.

Substrates

A chromogenic substrate for plasmin.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Streptococcus pneumoniae choline-binding protein E interaction with plasminogen/plasmin stimulates migration across the extracellular matrix.
Attali C, et al.
Infection and Immunity, 76(2), 466-476 (2008)
Min Wang et al.
International journal of biological macromolecules, 146, 781-789 (2019-11-16)
The heterologous nature of SAK, a thrombolytic drug, elicits high titers of neutralizing antibodies, which limits its clinical use. Here, we aim to establish a SAK mutant with equivalent activity to the wild type but reduced antigenicity, which may allow
Overexpression of aprE2, a Fibrinolytic Enzyme Gene from Bacillus subtilis CH3-5, in Escherichia coli and the Properties of AprE2.
Jeong SJ, et al.
Journal of microbiology and biotechnology, 24(7), 969-978 (2014)
Xiaoyu Xu et al.
BMC microbiology, 17(1), 178-178 (2017-08-20)
Porphyromonas gingivalis is a major causative pathogen of chronic periodontitis. Within the inflammatory microenvironment, there exists extreme pH values, elevated temperatures and oxidative stress. Pathogens adapt to these stressful environmental conditions by regulating the transcription of virulence genes, modifying themselves
Purification and characterization of a major fibrinolytic enzyme from Bacillus amyloliquefaciens MJ5-41 isolated from Meju
Jo HD, et al.
Journal of microbiology and biotechnology, 21(11), 1166-1173 (2011)

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