SRP0731

TRKA (G667C), Active

PRECISIO^® Kinase, recombinant

• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• recombinante: expressed in baculovirus infected Sf9 cells
• Ensayo: 70-95% (SDS-PAGE)
• Formulario: frozen liquid
• actividad específica: 20-30 nmol/min·mg (radiometric)
• mol peso: ~66 kDa
• concentración: 0.05 μg/μL
• nº de acceso: NM_002529
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... TRKA(4914)

Descripción

Aplicación

Useful for kinase assay.

Acciones bioquímicas o fisiológicas

Recombinant human TRKA (G667C) (440-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKA is a member of the trk proto-oncogene family and encodes a 140kDa, membrane-spanning protein tyrosine kinase that is the functional receptor for nerve growth factor (NGF). NGF elicits the rapid phosphorylation of gp140trk on tyrosine residues leading to increased c-Fos expression, DNA synthesis and morphologic transformation ⁽¹⁾. A decreased expression of TRKA on the striatal cholinergic neurons has been observed which may contribute, when it reaches a crucial threshold, to the death of cholinergic neurons observed in Alzheimer disease ⁽²⁾.

Reconstitución

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Almacenamiento y estabilidad

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Información legal

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable