SHC004H

MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Transduction Particles, High Titer

High Titer, shRNA sequence targeting tGFP

• Sinónimos: MISSION^®, MISSION^® Control Transduction Particles
• UNSPSC Code: 41106609
• NACRES: NA.51

Propiedades

• Quality Level: 200
• product line: MISSION^®
• concentration: ≥1x10⁹ VP/ml (via p24 assay)
• technique(s): capture ELISA: 10⁹ TU/mL using p24
• shipped in: dry ice
• storage temp.: −70°C

Descripción

General description

The MISSION^® TurboGFP^™ shRNA Control Transduction Particles contain an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Particles are useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁹ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

This high titer offering is recommended for customers: who need to utilize smaller volumes of virus, who have very difficult to transduce cell lines, or who need to transduce large numbers of cells.

When conducting experiments using MISSION^® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Components

High titer controls are provided as 10 x 20 μl aliquots.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboGFP is a trademark of Evrogen Co.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable