SAB4200621

Monoclonal Anti-NG2/CSPG4 antibody produced in mouse

clone 2164B6, purified from hybridoma cell culture

• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2164B6, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): flow cytometry: 2.5-5 μg/test using SK-Mel-28 cells.; immunofluorescence: 2.5-5 μg/mL using A375 cells.
• isotype: IgG1
• UniProt accession no.: Q6UVK1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CSPG4(1464)

Descripción

General description

Nerve/glial antigen 2 (NG2), a high molecular mass melanoma- associated antigen (HMW-MAA), is a large integral membrane, cell surface type I transmembrane protein that is covalently modified with chondroitin sulfate glycosaminoglycan.

Immunogen

a recombinant extracellular portion of human NG2 proteoglycan

Application

Monoclonal Anti-NG2/CSPG4 antibody produced in mouse used in flow cytometry and immunocytochemistry.

Biochem/physiol Actions

Nerve/glial antigen 2 (NG2) plays a central role in linking multiple oncogenic pathways required for malignant progression. NG2 expression in radial growth phase (RGP) human melanomas enables migration, protease activation and epithelial to mesenchymal transition, which is important in primary tumors for facilitating progression from a radial to vertical growth phase phenotype. It regulates sustained, high-level activation of key survival and growth pathways, integrin-regulated focal adhesion kinase (FAK), extracellular signal-regulated kinases 1 and 2 (ERK 1,2) and phosphoinositide 3-kinase (PI3K)/AKT pathways.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable