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R7131

Sigma-Aldrich

RPMI 1640 Amino Acids Solution (50×)

Without L-glutamine, sterile-filtered, BioReagent, suitable for cell culture

Sinónimos:

Amino Acid Supplement

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About This Item

UNSPSC Code:
12352209
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Categorías relacionadas

General description

RPMI 1640 Amino Acids Solution (50×) serves as an amino acid concentrate to supplement Roswell Park Memorial Institute medium (RPMI)-based mammalian cell culture media.

Application

RPMI 1640 Amino Acids Solution (50×) has been used as a supplement:
  • in M9 minimal medium to culture the bacterial strains, SX701 and JE116
  • to culture blood neutrophils from lactation cows in vitro
  • for amino acid stimulation in amino-acid-free Roswell Park Memorial Institute medium (RPMI) medium to grow HeLa cells

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supplement

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Nuno Carinhas et al.
BMC systems biology, 5, 34-34 (2011-03-01)
Stoichiometric models constitute the basic framework for fluxome quantification in the realm of metabolic engineering. A recurrent bottleneck, however, is the establishment of consistent stoichiometric models for the synthesis of recombinant proteins or viruses. Although optimization algorithms for in silico
M Garcia et al.
Journal of dairy science, 99(5), 3777-3783 (2016-03-14)
Glutamine is the preferred AA used by polymorphonuclear leukocytes (PMN) during the inflammatory response. However, the effect of other AA on bovine PMN response during inflammation and how this is altered by stage of lactation has not been fully elucidated.
G Ullman et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 368(1611), 20120025-20120025 (2012-12-26)
We have developed a method combining microfluidics, time-lapsed single-molecule microscopy and automated image analysis allowing for the observation of an excess of 3000 complete cell cycles of exponentially growing Escherichia coli cells per experiment. The method makes it possible to
D Kazyken et al.
The Journal of biological chemistry, 297(4), 101100-101100 (2021-08-22)
The mechanistic target of rapamycin (mTOR) complex 2 (mTORC2) signaling controls cell metabolism, promotes cell survival, and contributes to tumorigenesis, yet its upstream regulation remains poorly defined. Although considerable evidence supports the prevailing view that amino acids activate mTOR complex
Jakub Wiktor et al.
Nature, 597(7876), 426-429 (2021-09-03)
Homologous recombination is essential for the accurate repair of double-stranded DNA breaks (DSBs)1. Initially, the RecBCD complex2 resects the ends of the DSB into 3' single-stranded DNA on which a RecA filament assembles3. Next, the filament locates the homologous repair
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