R102-05N

Rat Epidermal Keratinocytes: REK, neonatal

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: rat skin (Sprague Dawley)
• Nivel de calidad: 100
• envase: pkg of 500,000 cells
• fabricante / nombre comercial: Cell Applications, Inc
• modo de crecimiento: Adherent
• morfología: Epidermal
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: allergies; cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

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REK from Cell Applications, Inc. provide a suitable model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology. It was shown that TGFβ1 treatment decreased c-Myc mRNA in REK which, taken together with the TGFβ1-induced growth arrest exhibited by keratinocytes and opposite results obtained in other cells, suggests a correlation between c-Myc mRNA expression and the mitogenic response (Nakai, 2008). Additionally, REK were used to demonstrate that pathological increases in keratinocyte sodium channels Nav1.1, Nav1.6, and Nav1.8 expression may contribute to pain by increasing epidermal ATP release, resulting in excessive activation of P2X receptors on primary sensory axons (Zhao, 2008). REK were also used to demonstrate that laminin 332 deposition is inhibited by ionizing radiation and, in combination with slower keratinocyte migration, can contribute to the delayed wound healing of irradiated skin (Jourdan, 2011).

Origen línea celular

Skin

Aplicación

Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response.

Componentes

Rat Keratinocyte Basal Medium that contains 10% FBS and 10% DMSO

Calidad

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Nota de preparación

• Primary culture, >500,000 cells in Rat Keratinocyte Basal Medium that contains 10% FBS and 10% DMSO
• These epithelial cells proliferate well when thawed and plated from cryopreservation, but they do not plate and proliferate after trypsinization

Rutina de subcultivo

Please refer to the RDF Culture Protocol.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable