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P8215

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use with fungal and yeast extracts, DMSO solution

Sinónimos:

Protease Inhibitor Mix, Protease inhibitor

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About This Item

Número CE:
200-664-3
Número MDL:
MFCD00677817
Código UNSPSC:
12352200
NACRES:
NA.54

Nombre del producto

Cóctel de inhibidores de proteasas, for use with fungal and yeast extracts, DMSO solution

Nivel de calidad

300

Formulario

DMSO solution

solubilidad

water: soluble

temp. de almacenamiento

−20°C

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Descripción general

The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast.

Especificidad

Inhibits serine, cysteine, aspartic, and metalloproteases.

Aplicación

Protease Inhibitor Cocktail has been used as a component in:
  • FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
  • potassium phosphate buffer (pbs) to digest chitin
  • extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis

Características y beneficios

  • Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts.
  • Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain.
  • Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage.
  • Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays.
  • Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results.

Componentes

AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM

Otras notas

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Cantidad

One mL is recommended for the inhibition of proteases extracted from 20 g of yeast.

Forma física

Solution in DMSO

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Pictogramas

Exclamation markEnvironment
GHS07,GHS09

Palabra de señalización

Warning

Frases de peligro

H302,H315,H319,H410

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

188.6 °F

Punto de inflamabilidad (°C)

87 °C

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Certificados de análisis (COA)

Lot/Batch Number
0000307845
0000307846
0000233228
0000233229

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Visite la Librería de documentos

Jin H Lee et al.
Virology, 378(2), 347-354 (2008-07-08)
In this study we have defined protein-protein interactions between the structural proteins of herpes simplex virus type 1 (HSV-1) using a LexA yeast two-hybrid system. The majority of the capsid, tegument and envelope proteins of HSV-1 were screened in a
Wei Xie et al.
Molecular biology of the cell, 20(14), 3317-3329 (2009-05-22)
Endoplasmic reticulum (ER) quality control mechanisms monitor the folding of nascent polypeptides of the secretory pathway. These are dynamic processes that retain folding proteins, promote the transport of conformationally mature proteins, and target misfolded proteins to ER-associated degradation (ERAD) pathways.
Takashi Kubota et al.
Molecular & cellular proteomics : MCP, 10(7), M110-M110 (2011-04-21)
Yeast cells lacking Ctf18, the major subunit of an alternative Replication Factor C complex, have multiple problems with genome stability. To understand the in vivo function of the Ctf18 complex, we analyzed chromatin composition in a ctf18Δ mutant using the
Elena Pérez-Nadales et al.
The Plant cell, 23(3), 1171-1185 (2011-03-29)
Fungal pathogenicity in plants requires a conserved mitogen-activated protein kinase (MAPK) cascade homologous to the yeast filamentous growth pathway. How this signaling cascade is activated during infection remains poorly understood. In the soil-borne vascular wilt fungus Fusarium oxysporum, the orthologous
A genome-wide screen reveals a role for the HIR histone chaperone complex in preventing mislocalization of budding yeast CENP-A
Ciftci-Yilmaz S, et al.
Genetics, 210(1), 203-218 (2018)
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Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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