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P1944

Sigma-Aldrich

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

Sinónimos:

phenol chloroform RNA extraction

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About This Item

Código UNSPSC:
12190000
NACRES:
NA.52
grado:
for molecular biology
Ensayo:
≥99.0%
aplicaciones:
life science and biopharma

grado

for molecular biology

Nivel de calidad

200

Ensayo

≥99.0%

Formulario

liquid

pH

4.3-4.7

aplicaciones

life science and biopharma

temp. de almacenamiento

2-8°C

Descripción general

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.

Aplicación

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
  • for the extraction and purification of RNA.
  • to isolate RNA from Arabidopsis siliques.
  • to extract proteins and DNA from polysomal and non-polysomal fractions.
  • in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Órganos de actuación

Liver,Kidney, Nervous system,Kidney,Liver,Skin

Código de clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

175.0 °F - closed cup

Punto de inflamabilidad (°C)

79.44 °C - closed cup

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
MKCW7584
MKCV5362
MKCV5361
MKCT1646
MKCT0359

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Visite la Librería de documentos

PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.
Rock C
Applied and Environmental Microbiology, 76(24), 8102-8109 (2010)
Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
Small untranslated RNA antitoxin in Bacillus subtilis.
Silvaggi JM
Journal of Bacteriology, 187(19), 6641-6650 (2005)
Autophagy controls resource allocation and protein storage accumulation in Arabidopsis seeds
Berardino JD, et al.
Journal of Experimental Botany, 69(6), 1403-1414 (2018)
Machine learning-assisted global DNA methylation fingerprint analysis for differentiating early-stage lung cancer from benign lung diseases
Lu D, et al.
Biosensors And Bioelectronics, 235, 115235-115235 (2023)
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