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Merck

P1063

Sigma-Aldrich

o-Fenilendiamina dihydrochloride

tablet, 60 mg substrate per tablet

Sinónimos:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

Fórmula lineal:
C6H4(NH2)2·2HCl
Número de CAS:
Peso molecular:
181.06
Beilstein:
3912045
Número CE:
Número MDL:
Código UNSPSC:
12352204
ID de la sustancia en PubChem:
NACRES:
NA.83

Nivel de calidad

Formulario

tablet

mp

258 °C (dec.) (lit.)

temp. de almacenamiento

2-8°C

cadena SMILES

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

Clave InChI

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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Descripción general

El diclorhidrato de o-fenilendiamina (OPD) es un sustrato cromógeno que puede utilizarse en procedimientos de enzimoinmunoanálisis de absorción (ELISA), ya que utiliza conjugados de peroxidasa de rábano picante. El sustrato rinde un producto final soluble que es de color naranja-marrón y puede leerse en el espectrofotómetro a 450 nm. La reacción del OPD puede detenerse con HCl 3N o H2SO4 3 M y leerse a 492 nm.
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Aplicación

o-Phenylenediamine dihydrochloride has been used as a stop solution for evaluating bovine serum albumin (BSA) antibodies via enzyme-linked immunosorbent assay (ELISA). It has also been used to measure specific antibodies in pig serum by ELISA.

Características y beneficios

Supplied as 50 or 100 tablets per box, individually wrapped with foil for easy storage, use, and safety.

Reconstitución

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Palabra de señalización

Warning

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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