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Key Documents

P0059

Sigma-Aldrich

Pro-carboxypeptidase B human

≥2.0 units/mg protein

Sinónimos:

Thrombin Activatable Fibrinolysis Inhibitor (TAFI)

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

Quality Level

specific activity

≥2.0 units/mg protein

mol wt

60 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CPB1(1360)

Biochem/physiol Actions

TAFI is supplied in its 60 kDa zymogen form. The N-terminal of TAFI contains four N-linked glycans and a plasminogen recognition sequence. Hydrolysis of the activation peptide by thrombin releases a 36-kDa active proteolytic domain homologous to tissue carboxypeptidase B. Complexation with thrombomodulin increases thrombin activation of TAFI 1250 fold. TAFI exerts it′s inhibition of fibrinolytic activity by binding to plasminogen and by cleaving C-terminal lysine residues of fibrin resulting in the reduction of plasmin and tPA binding sites on fibrin. Experiments in TAFI-deficient mice point to a regulatory role on complement C5a.

Unit Definition

One unit will hydrolyze 1 micromole of Hippuryl-L-Arginine per minute at pH 7.4 at 25 °C.

Physical form

Solution containing 20 mM Hepes and 150 mM Sodium chloride. pH 7.4

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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B N Bouma et al.
Journal of thrombosis and haemostasis : JTH, 1(7), 1566-1574 (2003-07-23)
Recently, a new inhibitor of fibrinolysis was described, which downregulated fibrinolysis after it was activated by thrombin, and was therefore named TAFI (thrombin-activatable fibrinolysis inhibitor; EC 3.4.17.20). TAFI turned out to be identical to the previously described proteins, procarboxypeptidase U
Toshihiko Nishimura et al.
Blood, 109(5), 1992-1997 (2006-11-16)
Plasma procarboxypeptidase B (proCPB) is activated by the endothelial thrombin-thrombomodulin [corrected] complex. Activated proCPB [corrected] (CPB) functions as a fibrinolysis inhibitor, but it may play a broader role by inactivating inflammatory mediators. To test this hypothesis, C5a-induced alveolitis was studied

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