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Merck

N5288

Sigma-Aldrich

Anti-NQO1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-DIA4, Anti-DT-diaphorase, Anti-Diaphorase 4, Anti-NAD (P)H:Menadione oxidoreductase 1, dioxin-inducible 1, Anti-NAD(P)H dehydrogenase, quinone 1, Anti-NMOR1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 2-5 μg using whole extract of human HeLa cells
indirect immunofluorescence: 2-5 μg/mL using human HeLa cells
western blot: 0.1-0.2 μg/mL using whole extract of rat NRK cells
western blot: 0.2-0.5 μg/mL using whole extract of mouse 3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NQO1(1728)
mouse ... Nqo1(18104)
rat ... Nqo1(24314)

General description

NADPH:quinone oxidoreductase (NQO1) is an FAD containing electron reductase that regulates the detoxification of quinones obtained from the phenolic metabolites of benzene. It is a homodimeric protein that can bind either of the reducing cofactors; NADPH or NADH . NQO1 regulates chemoprotection, neuroprotection, antitumor quinones and antioxidative defense mechanisms . NQO1 may be overexpressed in breast, colon, pulmonary and hepatic tumors.
NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) is a 2-electron reductase. NQO1 is a FAD-containing protein that exists as a homodimer. It is ubiquitously expressed.

Specificity

Anti-NQO1 (C-terminal) antibody is specific for rat, mouse, and human NQO1. The immunizing peptide specifically inhibits the detection of NQO1 band by immunoblotting.

Immunogen

Synthetic peptide sequence corresponding to amino acid residues 261-274 of human NQO1, with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.
synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Application

Anti-NQO1 (C-terminal) antibody may be used in immunological applications such as immunoprecipitation, immunofluorescence and immunoblotting (approximately 28 kDa).
Anti-NQO1 (C-terminal) antibody produced in rabbit has been used in:
  • western blotting
  • immunoprecipitation
  • immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) can bioactivate certain antitumor quinones. NQO1 has a role in antioxidant defense via the generation of antioxidant forms of ubiquinone and vitamin E. High levels of NQO1 gene expression has been observed in liver and lung tumors as compared to normal tissues of the same origin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

M Belinsky et al.
Cancer metastasis reviews, 12(2), 103-117 (1993-06-01)
NAD(P)H:Quinone Oxidoreductase1 (NQO1) also known as DT-diaphorase is a flavoprotein that catalyzes the two-electron reduction of quinones, quinone imines and azo-dyes and thereby protects cells against mutagenicity and carcinogenicity resulting from free radicals and toxic oxygen metabolites generated by the
Antiproliferative and antimetabolic effects behind the anticancer property of fermented wheat germ extract
Otto C, et al.
BMC Complementary and Alternative Medicine, 16(1), 160-160 (2016)
Yanxia Liu et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(12), 5205-5210 (2007-03-16)
The antioxidant response element (ARE) is a cis-acting regulatory enhancer element found in the 5' flanking region of many phase II detoxification enzymes. Up-regulation of ARE-dependent target genes is known to have neuroprotective effects; yet, the mechanism of activation is
NAD(P)H:quinone oxidoreductase 1 (NQO1): chemoprotection, bioactivation, gene regulation and genetic polymorphisms
Ross D, et al.
Chemico-Biological Interactions, 77-97 (2000)
Jennifer M McCracken et al.
Gene expression, 17(3), 187-205 (2017-02-25)
Biological differences exist between strains of laboratory mice, and it is becoming increasingly evident that there are differences between substrains. In the C57BL/6 mouse, the primary substrains are called 6J and 6N. Previous studies have demonstrated that 6J and 6N

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