N3393

Naphthol Blue Black

BioReagent, suitable for electrophoresis

• Sinónimos: Acid Black 1, Amido Black 10B, Buffalo Black NBR
• Fórmula empírica (notación de Hill): C₂₂H₁₄N₆Na₂O₉S₂
• Número de CAS: 1064-48-8
• Peso molecular: 616.49
• Colour Index Number: 20470
• Beilstein/REAXYS Number: 5374263
• EC Number: 213-903-1
• MDL number: MFCD00004017
• UNSPSC Code: 41105322
• PubChem Substance ID: 24897642
• NACRES: NA.32

Propiedades

• product line: BioReagent
• Quality Level: 200
• form: powder
• composition: Dye content, ~85%
• technique(s): protein staining: suitable
• impurities: ≤10% water
• suitability: suitable for electrophoresis
• SMILES string: [Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;
• InChI key: AOMZHDJXSYHPKS-DROYEMJCSA-L

Descripción

Application

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.

Biochem/physiol Actions

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1B
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves