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MTOX1010

Sigma-Aldrich

HepaRG™ 5F Control Cells

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

Sinónimos:

Human hepatocyte cells

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.24
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

HepaRG™ 5F Control Cells, 1 vial

biological source

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

Quality Level

200

form

liquid

storage temp.

−196°C

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
This product consists of ZFN engineered HepaRG 5F clone control cells. They are intended for use as both control cells for HepaRG™ knockout cells as well as for a wide variety of liver cell based assays. Hepa RG cells display hepatocyte-like functions and can be a replacement to primary human hepatocytes in a number of drug metabolism, disposition and genotoxicity studies.[1][2]

Application

HepaRG™ 5F Control Cells has been used in a gene expression assay to study the effect of pregnane X receptor on the induction of human cytochrome P450 3A4 by the irreversible myeloperoxidase inactivator PF-06282999.[3]
See technical bulletins for detailed protocols

Features and Benefits

Sigma′s HepaRG 5F Clone exhibits improved growth characteristics and enhanced functionality, expressing a large panel of drug metabolism enzymes; including cytochrome P450 enzymes CYP1A2, CYP2B6, CYP2C8/9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4, nuclear receptors AhR, PXR, CAR, LXR, and FXR, and phase II enzymes UGT, SULT, NAT and GST.

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

Legal Information

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLBS2876V
SLBL7390V

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Assessment of the genotoxic potential of indirect chemical mutagens in HepaRG cells by the comet and the cytokinesis-block micronucleus assays.
Le Hegarat, Ludovic, et al.
Mutagenesis, 25, 555-560 (2010)
Daochuan Li et al.
Biochemical pharmacology, 98(1), 190-202 (2015-08-16)
The constitutive androstane receptor (CAR) modulates the transcription of numerous genes involving drug metabolism, energy homeostasis, and cell proliferation. Most functions of CAR however were defined from animal studies. Given the known species difference of CAR and the significant cross-talk
The HepaRG cell line: a unique in vitro tool for understanding drug metabolism and toxicology in human.
Andersson, Tommy
Expert Opinion on Drug Metabolism & Toxicology, 8, 909-920 (2012)
Induction of human cytochrome P450 3A4 by the irreversible myeloperoxidase inactivator PF-06282999 is mediated by the pregnane X receptor.
Moscovitz, Jamie E., et al.
Xenobiotica, 1-9 (2017)
Tristan M Sissung et al.
Molecular pharmacology, 96(2), 158-167 (2019-06-09)
Mithramycin demonstrates preclinical anticancer activity, but its therapeutic dose is limited by the development of hepatotoxicity that remains poorly characterized. A pharmacogenomics characterization of mithramycin-induced transaminitis revealed that hepatotoxicity is associated with germline variants in genes involved in bile disposition:
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The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic circulation.

Questions

  1. HepaRG™ 5Fコントロール細胞の可能継代回数、増殖速度についてお尋ねしたいです。

    1 answer

    1. TS Answer: the MTOX1010 cells are intended for use as both control cells for HepaRG™ knockout cells as well as for a wide variety of liver cell based assays. As such we do not have a passage number limit but a minimum culturing period. Cells should be differentiated for a minimum of 14 days, exchanging medium twice weekly, before our suggested assays (see protocol attached).

      Please note that the MTOX1010 HepaRG™ 5F Control Cells as well as the MTOX1011 PXR Knockout HepaRG™ Cells are patented, their use is strictly limited, and you are not allowed to passage them further. If you wish to do so, you would need to purchase a license.

      HepaRG™ 5F and Knockout Limited Use License

      These cells are derived from HepaRG® cells and are protected by patents; by opening this package, you agree to abide the terms of this Limited Use License as follows. You will consider the cells as a disposable product to be destroyed upon conclusion of a study or experiment; will use them only for in-vitro experiments and only at the facility of their receipt; will not propagate, amplify, reproduce, clone, or make any other use of them after a study is concluded; will not produce or manufacture commercial products or cDNA libraries or cell stock from the cells; will not use them for any study of a duration exceeding 20 days; and you will not license, resell or transfer them to anyone outside your organization for any reason. If you are unwilling to accept the terms of this LIMITED USE LICENSE, do not use the cells and return them to Sigma-aldrich for credit. Violators of this LIMITED USE LICENSE will be prosecuted to the fullest extent of the law.

      You can find additional information via the following link:
      https://www.sigmaaldrich.com/life-science/adme-tox-assays/license-agreements.html

      The Technical Bulletin link below describes the confluency determination and growth rate:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/530/047/mtox1010bul.pdf

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