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M8662

Sigma-Aldrich

Aceite de inmersión

PCR Reagent

Sinónimos:

Mineral oil for PCR

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About This Item

Número de CAS:
8042-47-5
Número CE:
232-455-8
Número MDL:
MFCD00131611
Código UNSPSC:
12181504
NACRES:
NA.52
grado:
PCR Reagent
técnicas:
PCR: suitable

grado

PCR Reagent

Nivel de calidad

100

Formulario

liquid

envase

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

técnicas

PCR: suitable

color

colorless

índice de refracción

n20/D 1.467 (lit.)

densidad

0.84 g/mL at 25 °C (lit.)

actividad extraña

DNase, RNase, protease, none detected

temp. de almacenamiento

room temp

Clave InChI

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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Descripción general

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

Aplicación

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform

Características y beneficios

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

No data available

Punto de inflamabilidad (°C)

No data available

Equipo de protección personal

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
MKCW2105
MKCN0398
MKCK1218
MKCF5776
MKCC0168

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Hsin-I Jen et al.
eLife, 8 (2019-04-30)
The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
Ivan K Dimov et al.
Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Hartmut Kaiser et al.
Plant physiology, 143(2), 1068-1077 (2006-12-13)
The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
Decagram scale production of deuterated mineral oil and polydecene as solvents for polymer studies in neutron scattering
Klenner MA, et al.
Open Journal of Polymer Chemistry, 11(31), 4986-4994 (2020)
Neus Bota-Rabassedas et al.
Cell reports, 35(3), 109009-109009 (2021-04-22)
Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
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Protocolos

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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