Saltar al contenido
Merck
Todas las fotos(4)

Key Documents

View All Documentation

M7133

Sigma-Aldrich

4-Methylumbelliferyl sulfate potassium salt

sulfatase substrate

Sinónimos:

Potassium 4-methylumbelliferyl sulfate

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(4)

About This Item

Fórmula empírica (notación de Hill):
C10H7KO6S
Número de CAS:
15220-11-8
Peso molecular:
294.32
Beilstein/REAXYS Number:
3803203
EC Number:
239-272-2
MDL number:
MFCD00016970
UNSPSC Code:
12352204
PubChem Substance ID:
24897141
NACRES:
NA.32

Quality Level

200

assay

≥99% (HPLC)

form

powder

solubility

water: 5 mg/mL, clear, colorless

fluorescence

λex 334 nm; λem 370 nm (pH 10.4)
λex 360 nm; λem 449 nm (Reaction products)

storage temp.

−20°C

SMILES string

[K+].CC1=CC(=O)Oc2cc(OS([O-])(=O)=O)ccc12

InChI

1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1

InChI key

CSOCSPXOODWGLJ-UHFFFAOYSA-M

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

4-Methylumbelliferyl sulfate (4MUS), a general substrate, is a polar metabolite of 4-methylumbelliferone (4MU).

Application

4-Methylumbelliferyl sulfate potassium salt has been used as a substrate in coupled fluorescence sulfotransferase assay to regenerate 3′-phosphoadenosine 5′-phosphosulfate (PAPS) from 3′-phosphoadenosine 5′-phosphate for cerebroside sulfotransferase (CST) enzyme activity measurements. It has also been used as a substrate in arylsulfatase assay/4-methylumbelliferyl sulfate (4-MUS) assay to test the enzymatic activity of the protein-bead complex.

Biochem/physiol Actions

4-Methylumbelliferyl sulfate (4MUS) is capable of undergoing desulfation and involve in futile cycling with 4MU. It can be utilized to measure the arylsulfatase activity of several sulfatases.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

A M Hassen et al.
Drug metabolism and disposition: the biological fate of chemicals, 24(7), 792-798 (1996-07-01)
The uptake of estrone sulfate (E1S; 1 to 400 microM), harmol sulfate (HS; 5 to 900 microM), and 4-methylumbelliferyl sulfate (4MUS; 5 to 1000 microM) was investigated in isolated rat hepatocytes in the presence or absence of inhibitors. Uptake of
O P van Diggelen et al.
Journal of inherited metabolic disease, 12(3), 273-280 (1989-01-01)
Arylsulphatase C (ASC) activity in leukocytes and fibroblasts measured with 4-methylumbelliferylsulphate, is caused by at least two genetically different sulphatases. One of these is steroid sulphatase (STS). Depending on the substrate concentration, about 10-50% of the ASC activity in leukocytes
C L Zimmerman et al.
Journal of chromatography, 563(1), 83-94 (1991-01-18)
A direct high-performance liquid chromatographic (HPLC) assay was developed for the separation and determination of 4-methylumbelliferone (4MU) and its glucuronide (MUG) and sulfate (MUS) conjugates in the cell-free perfusate ("plasma") from in situ perfused rat intestine-liver preparation. In addition, a
J Chen et al.
The Journal of pharmacology and experimental therapeutics, 280(1), 24-31 (1997-01-01)
The vascularly perfused rat intestine and liver preparations were used to examine the effect of flow (8 and 10 ml/min) on the sequential metabolism of 4-methylumbelliferone (4MU), which forms primarily the glucuronide conjugate (4MUG) in intestine and the sulfate conjugate
Lihua Wang-Eckhardt et al.
Cells, 10(12) (2021-12-25)
Sulfatide synthesis in the human renal cancer cell line SMKT-R3 was strongly inhibited in the presence of low µM concentrations of AG-205, a progesterone receptor membrane component 1 (PGRMC1) antagonist. This was also the case in Chinese hamster ovary (CHO)
Ver todos los artículos relacionados

Artículos

Drug Conjugate Analysis using β-Glucuronidases

Drug conjugate analysis and the enzymatic hydrolysis of glucuronides

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico