M6570

Monoclonal Anti-Melan-A antibody produced in mouse

clone A103, purified from hybridoma cell culture

• Sinónimos: Anti-MART1
• Número MDL: MFCD01633630
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: A103, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~22 kDa
• reactividad de especies: human, canine
• concentración: ~2 mg/mL
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using total cell extract of SKMEL19 cells ()
• isotipo: IgG1
• Nº de acceso UniProt: Q16655
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... MLANA(2315)

Descripción

Descripción general

Melan-A is also known as MART-1 (melanoma antigen recognized by T-cells 1). It is a transmembrane protein which is hydrophobic in nature. Melan-A is expressed in melanocytes and melanoma tumors.[1]

Monoclonal Anti-Melan-A (mouse IgG1 isotype) is derived from the hybridoma A103 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with a human recombinant Melan-A protein. Melan-A (also known as MART1) is a melanoma antigen recognized by T cells. The protein is expressed mainly in cells and tumors of melanocytic lineage and thus belongs to the melanocyte differentiation markers.

Inmunógeno

recombinant human Melan-A protein.

Aplicación

Anti-Melan-A antibody, Mouse monoclonal has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• immunoprecipitation
• immunohistochemistry

Acciones bioquímicas o fisiológicas

Melan-A/MART1 may have a role in pigmentation since its expression pattern and sub cellular distribution is similar to other melanosomal proteins and correlates with melanin content. Melan-A is important for Pmel17 activity, forms a complex with it, and affects its expression, stability, trafficking, and processing that is required for melanosome structure and maturation. Thus, Melan-A is important for Pmel17 function and therefore it is important for mammalian pigmentation.

Descripción de destino

Melan-A is a melanoma antigen recognized by T cells.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)