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Merck

LY0100

Sigma-Aldrich

Lysozyme Activity Kit

sufficient for 100 tests

Sinónimos:

Lysozyme assay kit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

usage

sufficient for 100 tests

Quality Level

storage temp.

2-8°C

General description

The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay to detect lysozyme activity uses Micrococcus lysodeikticus cells as the substrate. Lysozyme activity results in the lysis of the Micrococcus lysodeikticus cells. During incubation of the lysozyme sample and substrate, the reaction is followed by monitoring the decrease in absorbance at 450 nm.

Application

Lysozyme Activity Kit has been used to measure the catalytic activity of native lysozyme, lysozyme purified from chicken egg white and lysozyme from pilocarpine-stimulated saliva.

Biochem/physiol Actions

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.

Storage and Stability

The Reaction Buffer is stable for at least 2 years at 2–8 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • L6876Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg proteinSDS

  • Lysozyme Reaction Buffer

  • M3770Micrococcus lysodeikticus ATCC No. 4698, suitable for substrate for the assay of lysozyme, lyophilized cellsSDS

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Silica Nanopollens Enhance Adhesion for Long-Term Bacterial Inhibition.
Song, H., et al.
Journal of the American Chemical Society, 138, 6455-6462 (2016)
Paula Chequer Gouveia Mól et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1044-1045, 17-23 (2017-01-09)
In this study, a supermacroporous polyacrylamide cryogel was produced by cryo-polymerization and activated with Tris(hydroxymethyl)aminomethane (Tris-cryogel) to be applied as an affinity ligand for a one step purification of lysozyme (LYZ), directly from chicken egg white (EW). The Tris-cryogel presented
Tao Wang et al.
Chemical science, 7(5), 3234-3239 (2016-05-01)
Water-soluble allyl sulfones provide convenient site-specific disulfide rebridging of native proteins and cyclic peptides. The site-selective functionalization of (a) the peptide hormone somatostatin, (b) the interchain disulfide of bovine insulin and (c) functionalization of the proteins GFP and lysozyme with
Dan Bi Lee et al.
Scientific reports, 8(1), 2541-2541 (2018-02-09)
Time-resolved serial femtosecond crystallography with X-ray free electron laser (XFEL) holds the potential to view fast reactions occurring at near-physiological temperature. However, production and characterization of homogeneous micron-sized protein crystals at high density remain a bottleneck, due to the lack
Ji-Won Jang et al.
Chemosphere, 260, 127611-127611 (2020-08-08)
Mercury (Hg) is known as a highly toxic heavy metal, and its toxicity varies depending on its form due to different toxicokinetics between inorganic and organic Hg. Limited information on comparison of Hg toxicity concerning its chemical form by oral

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