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Merck

L4777

Sigma-Aldrich

Lipase acrylic resin

greener alternative

≥5,000 U/g, recombinant, expressed in Aspergillus niger

Sinónimos:

Novozyme 435

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad

recombinante

expressed in Aspergillus niger

Nivel de calidad

Formulario

solid

actividad específica

≥5,000 U/g

características de los productos alternativos más sostenibles

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Matriz

macroporous acrylic resin

aplicaciones

diagnostic assay manufacturing

categoría alternativa más sostenible

temp. de almacenamiento

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

Clave InChI

QWZUIMCIEOCSJF-CHHCPSLASA-N

Descripción general

Lipase from produced by submerged fermentation of a genetically modified Aspergillus niger microorganism and adsorbed on a macroporous resin.
Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.[1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.

Aplicación

Immobilized preparation of a thermostable lipase, particularly useful in the synthesis of esters and amides, and has a broad substrate specificity.
Lipase acrylic resin has been used:
  • as a catalyst to carry out the esterification of pLACT with butyric acid[2]
  • as an additive in fully hydrogenated canola oil (FHCO) and high oleic sunflower oil (HOSO) blend for enzymatic interesterification[3]
  • to study its effect on the degree of oleic acid conversion upon esterification[4]

Acciones bioquímicas o fisiológicas

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries.[1] It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.[5] Candida antarctica lipase B is considered as an important enzyme due to its use in esterification, hydrolysis, aminolysis and transesterification.[4]

Otras notas

The material may be reused for identical reactions. It is not recommended for use in different reactions due to the probability of cross-contamination and loss of activity. There is no estimation as to the number of times the product may be used.

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Certificados de análisis (COA)

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Visite la Librería de documentos

Covalent immobilization of Candida antarctica lipase B on nanopolystyrene and its application to microwave-assisted esterification
Bukhari A, et al.
Chinese Journal of Catalysis, 35(9), 1555-1564 (2014)
Gurpur Rakesh D Prabhu et al.
Nature protocols, 15(3), 925-990 (2020-01-31)
Since the advent of modern science, researchers have had to rely on their technical skills or the support of specialized workshops to construct analytical instruments. The notion of the 'fourth industrial revolution' promotes construction of customized systems by individuals using
Filippo Fabbri et al.
International journal of molecular sciences, 22(16) (2021-08-28)
In the present work, different hydrolases were adsorbed onto polypropylene beads to investigate their activity both in short-esters and polyesters synthesis. The software MODDE® Pro 13 (Sartorius) was used to develop a full-factorial design of experiments (DoE) to analyse the
Biocatalyzed approach for the surface functionalization of poly (L-lactic acid) films using hydrolytic enzymes
Pellis A, et al.
Biotechnology Journal, 10(11), 1739-1749 (2015)
Marine fungal and bacterial isolates for lipase production: A comparative study
Advances in Food and Nutrition Research, 78, 71-94 (2016)

Questions

1–6 of 6 Questions  
  1. Hello. I would like to ask if this lipase is Lipase B from Candida Antarctica, as this is not specified in the SDS or the specification sheet.

    1 answer
    1. This product is produced by submerged fermentation of a genetically modified Aspergillus niger microorganism and adsorbed on a macroporous resin.

      Helpful?

  2. Is the supported enzyme reusable, and if so in which conditions?

    1 answer
    1. This product is supplied by Novozymes and is commercially available as Novozym 435. The material may be reused for identical reactions. It is not recommended for use in different reactions due to the probability of cross-contamination and loss of activity. There is no estimation as to the number of times the product may be used. The link below may be useful in regeneration. This information has not been validated.

      https://www.researchgate.net/figure/Regeneration-of-deactivated-Novozyme-435_fig7_313863272

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What are the components of Product L4777, Lipase acrylic resin, and what are the optimal recommended conditions?

    1 answer
    1. This product consists of a thermostable lipase enzyme immobilized on a macroporous acrylic bead resin. The bead-shaped particles have a diameter in the range of 0.3-0.9 mm. The resin will be compatible with stearyl alcohol at temperatures of 40-60°C, which is the normal operating temperature of the enzyme. This immobilized enzyme can be used in hexane with no adverse effects.

      Helpful?

  5. What solvents are most compatible with Product L4777, Lipase acrylic resin?

    1 answer
    1. This resin works well in solvent-free reactions, as well as in inert water immiscible solvents such as petroleum ether, n-hexane and n-heptane. If the reaction being catalyzed is hydrolysis of an ester, addition of water to the reaction mix is recommended. For other reactions water is likely not required, and in some cases would actually be detrimental.

      Helpful?

  6. What is the substrate specificity of Product L4777, Lipase acrylic resin?

    1 answer
    1. Lipase acrylic resin has a broad substrate specificity. It promotes reactions between a wide range of primary and secondary alcohols and carboxylic acids.

      Helpful?

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