L1378

L-Lactic Dehydrogenase from bovine muscle

Type X, ammonium sulfate suspension, ≥600 units/mg protein

• Sinónimos: (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD, Lactate
• Número de CAS: 9001-60-9
• Comisión internacional de enzimas: 1.1.1.27 (BRENDA, IUBMB)
• Número CE: 232-617-8
• Número MDL: MFCD00131466
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bovine muscle
• Nivel de calidad: 200
• tpo: Type X
• Ensayo: 3.0—25.0 mg protein/mL (biuret)
• Formulario: ammonium sulfate suspension
• actividad específica: ≥600 units/mg protein
• fabricante / nombre comercial: Sigma-Aldrich
• técnicas: activity assay: suitable
• color: white
• pH: 7.5
• Nº de acceso UniProt: P19858; Q5E9B1
• aplicaciones: life science and biopharma
• actividad extraña: pyruvate kinase ≤0.01%
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: cow ... LDHA(281274) , LDHB(281275)

Descripción

Descripción general

Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.

Aplicación

L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.

Acciones bioquímicas o fisiológicas

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.

Definición de unidad

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Forma física

Crystalline suspension in 2.4 M (NH₄)₂SO₄ solution, pH 6.0

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)