I5260

Anti-Human IgG (Fab specific) antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Fab-Specific Antibody
• Número MDL: MFCD00162431
• Código UNSPSC: 12352203
• NACRES: NA.46

Propiedades

• origen biológico: goat
• Nivel de calidad: 300
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: secondary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• técnicas: indirect ELISA: 1:50,000; quantitative precipitin assay: 2.0 mg/mL
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Anti-Human IgG (Fab specific) antiserum is produced in goat using purified human IgG Fab fragment as the immunogen. Affinity isolated antibody is obtained from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. IgG is a larger molecule with a molecular weight of 150 kilo daltons (kDa). IgG has two light chains (L) and two heavy chains (H). The light chain can be either lambda (λ) or kappa (κ) chain. Functionally, immunoglobulins have constant region and variable region. Light chain has one variable region in the N-terminal VL and followed by one constant domain CL. The light chain is approximately about 25 kDa. The heavy chain has one variable region in the N-terminal and followed by three constant domains. In between the first (C1H) and second constant (C2H) domain in the spacer hinge region which connects both the heavy chains. Each heavy chain is about 55 kDa.

Especificidad

Goat anti-human IgG (Fab specific) antibody reacts specifically with Fab fragment of human IgG and shows no reactivity for Fc fragment of human IgG. This product has no interspecies cross reactivity for mouse and rat IgG.

Inmunógeno

Purified human IgG Fab fragment.

Aplicación

Anti-Human IgG (Fab specific) antibody produced in goat is effective as a second antibody reagent in immunoassay procedures. It may be used:

• as starting material for conjugates using enzymes or fluorescent dyes
• in enzyme linked immunosorbent assay
• as a cross-linking antibody in internalisation of ¹²⁵I-IgG by streptolysin-O-permeabilised cells
• as capture antibody in enzyme-linked immunosorbent assay (ELISA) for detection and semiquantitation of Fab
• in electrophoresis and immunoelectrophoresis

Acciones bioquímicas o fisiológicas

Enzymatic cleavage of IgG yields antigen-binding (Fab) and effector activating (Fc) fragments. IgG treatment with papain produces two Fab fragments and one Fc fragment. IgG treatment with pepsin produces (Fab′)₂ fragment alone. IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-human IgG (Fab specific) antibody can be used in Ouchterlony double diffusion. Due to lack of interspecies cross reactivity to mouse or rat ascites fluids, this antibody is also ideal for screening human monoclonal antibodies produced by hybridoma cells grown in vivo.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable