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Merck

I4401

Sigma-Aldrich

Interferon-αA/D human

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Sinónimos:

IFN-αA/D

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

human

Nivel de calidad

recombinante

expressed in E. coli

Ensayo

≥95%

Formulario

buffered aqueous solution

mol peso

19 kDa

envase

pkg of 100K units

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

color

colorless

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... IFNA2(3440)

Aplicación

Interferon-αA/D human has been used:
  • for identification of coronavirus replicase inhibitors
  • in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication
  • in combination with vitamin D to check reduction in HCV protein production in cell culture

Acciones bioquímicas o fisiológicas

Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.
This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Forma física

Solution in phosphate buffered saline containing 0.1% bovine serum albumin

Nota de análisis

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV). A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves


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C Lauber et al.
Genes and immunity, 16(6), 414-421 (2015-06-13)
The IFNL4 gene is negatively associated with spontaneous and treatment-induced clearance of hepatitis C virus infection. The activity of IFNλ4 has an important causal role in the pathogenesis, but the molecular details are not fully understood. One possible reason for
Pathompong Bowornruangrit et al.
Medical sciences (Basel, Switzerland), 10(1) (2022-03-25)
Autophagy is a known mechanism of cells under internal stress that regulates cellular function via internal protein recycling and the cleaning up of debris, leading to healthy live cells. However, the stimulation of autophagy by external factors such as chemical
Tobias Hertzig et al.
The Journal of general virology, 85(Pt 6), 1717-1725 (2004-05-29)
A previously unknown coronavirus (CoV) is the aetiological agent causing severe acute respiratory syndrome (SARS), for which an effective antiviral treatment is urgently needed. To enable the rapid and biosafe identification of coronavirus replicase inhibitors, we have generated a non-cytopathic
Jun Kang et al.
Journal of virology, 95(3) (2020-11-06)
Human enterovirus D68 (EV-D68) has received considerable attention recently as a global reemergent pathogen because it causes severe respiratory tract infections and acute flaccid myelitis (AFM). The nonstructural protein 2A protease (2Apro) of EVs, which functions in the cleavage of
Esther Arnaiz et al.
Frontiers in oncology, 11, 779739-779739 (2021-12-14)
Hypoxia is a common phenomenon in solid tumours strongly linked to the hallmarks of cancer. Hypoxia promotes local immunosuppression and downregulates type I interferon (IFN) expression and signalling, which contribute to the success of many cancer therapies. Double-stranded RNA (dsRNA)

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