GE28-9909-45
Superdex® 200 Increase 5/150 GL
Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size
Sinónimos:
Size-exclusion chromatography column
About This Item
Productos recomendados
material
glass column
polyethylene filter
product line
Superdex®
mol wt
10000-600000 Da (globular proteins)
manufacturer/tradename
Cytiva 28-9909-45
packaging
1 ea of
parameter
<0.75 mL/min flow rate
<0.75 ml/min flow rate
<3.0 Mpa (725 psi)
4-50 μL sample volume
bed volume
~3 mL
column L × I.D.
15 cm × 5 mm
matrix
agarose dextran composite
polymeric
matrix active group
phenyl phase
particle size
8.6 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
Categorías relacionadas
General description
Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.
Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.
Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.
This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.
Features and Benefits
- Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
- Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
- High flow rates: rigid beads give excellent pressure/flow properties.
Suitability
Storage and Stability
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Artículos
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Protocolos
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.
Contenido relacionado
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico