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Key Documents

GE28-9909-45

Sigma-Aldrich

Superdex® 200 Increase 5/150 GL

Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size

Sinónimos:

Size-exclusion chromatography column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

glass column
polyethylene filter

product line

Superdex®

mol wt

10000-600000 Da (globular proteins)

manufacturer/tradename

Cytiva 28-9909-45

packaging

1 ea of

parameter

<0.75 mL/min flow rate
<0.75 ml/min flow rate
<3.0 Mpa (725 psi)
4-50 μL sample volume

bed volume

~3 mL

column L × I.D.

15 cm × 5 mm

matrix

agarose dextran composite
polymeric

matrix active group

phenyl phase

particle size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

General description

Superdex™ 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.

Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.

Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.

Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.

This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Features and Benefits

  • Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
  • Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
  • High flow rates: rigid beads give excellent pressure/flow properties.

Suitability

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
Well suited for rapid purity checks and quick screenings of experimental conditions.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

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Flame

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Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Protocolos

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

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Contenido relacionado

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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