GE25-6600-22
GenomiPhi™ HY Kit
Cytiva 25-6600-22, sufficient for 25 reactions
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
usage
sufficient for 25 reactions
manufacturer/tradename
Cytiva 25-6600-22
availability
not available in Japan
shipped in
dry ice
storage temp.
−70°C
General description
illustra™ GenomiPhi™ HY DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 50 μg yield from nanogram amounts of DNA sample.
The illustra™ GenomiPhi™ HY DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for midi-scale whole genome amplification by isothermal strand displacement amplification.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.
The illustra™ GenomiPhi™ HY DNA Amplification Kit procedure is quick, simple and does not require a thermal cycler. A typical DNA yield of 40 to 50 μg DNA can be achieved in four h with little hands-on time. The average product length is over 10 kb. The starting material for GenomiPhi™ reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. The kit was verified with DNA from various clinical samples including blood and buccal swabs.
GenomiPhi™ amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archiving.
The illustra™ GenomiPhi™ HY DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for midi-scale whole genome amplification by isothermal strand displacement amplification.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.
The illustra™ GenomiPhi™ HY DNA Amplification Kit procedure is quick, simple and does not require a thermal cycler. A typical DNA yield of 40 to 50 μg DNA can be achieved in four h with little hands-on time. The average product length is over 10 kb. The starting material for GenomiPhi™ reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. The kit was verified with DNA from various clinical samples including blood and buccal swabs.
GenomiPhi™ amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archiving.
Application
For midi-scale whole genome amplification by isothermal strand displacement amplification; typical downstream applications are genotyping (SNP, STR, array CGH) and DNA archiving.
Features and Benefits
- Yields high-quality DNA (e.g., for high-throughput genotyping, hybridization, and DNA archival).
- Outperforms PCR-based whole genome amplification techniques.
- Quick and simple automation-friendly protocol; no thermal cycler required.
- No template-independent, background amplification product.
- Representative isothermal amplification of the whole genome.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
GenomiPhi
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
GenomiPhi is a trademark of Cytiva
illustra is a trademark of Cytiva
Storage Class
12 - Non Combustible Liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Haematologica, 98(4), 635-639 (2013-01-26)
The BRAF-V600E mutation defines genetically hairy cell leukemia among B-cell leukemias and lymphomas. In solid tumors, BRAF-V600E is known to aberrantly activate the oncogenic MEK-ERK pathway, and targeted BRAF and/or MEK inhibitors have shown remarkable efficacy in clinical trials in
Genome biology, 14(10), R113-R113 (2013-10-24)
Melanoma is the most deadly form of skin cancer. Expression of oncogenic BRAF or NRAS, which are frequently mutated in human melanomas, promote the formation of nevi but are not sufficient for tumorigenesis. Even with germline mutated p53, these engineered
Proceedings of the National Academy of Sciences of the United States of America, 110(26), E2390-E2399 (2013-06-12)
The "dark matter of life" describes microbes and even entire divisions of bacterial phyla that have evaded cultivation and have yet to be sequenced. We present a genome from the globally distributed but elusive candidate phylum TM6 and uncover its
Ecology and evolution, 2(3), 550-561 (2012-07-24)
It is difficult to predict how current climate change will affect wildlife species adapted to a tropical rainforest environment. Understanding how population dynamics fluctuated in such species throughout periods of past climatic change can provide insight into this issue. The
Genome research, 23(5), 878-888 (2013-03-16)
The majority of microbial genomic diversity remains unexplored. This is largely due to our inability to culture most microorganisms in isolation, which is a prerequisite for traditional genome sequencing. Single-cell sequencing has allowed researchers to circumvent this limitation. DNA is
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico