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GE17-5132-02

Glutathione Sepharose 4 Fast Flow

Cytiva 17-5132-02, pack of 100 mL

Sinónimos:

Sepharose 4 Fast Flow

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-5132-02

matrix

highly cross-linked 4% agarose

average diameter

90 μm

cleaning

3-12

working range

3-12

capacity

>10 mg/mL binding capacity

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.

Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose 4 Fast Flow provides good flow properties and is excellent for scaling up.

Features and Benefits

  • Fast, one-step purification of GST-tagged proteins
  • Mild elution conditions preserving protein antigenicity and function
  • For scale-up due to high flow properties
  • Glutathione coupled to Sepharose 4 Fast Flow via a 10-carbon spacer arm

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

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Protocolos

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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