G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

• Sinónimos: Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH
• Número de CAS: 9001-40-5
• Comisión internacional de enzimas: 1.1.1.49 (BRENDA, IUBMB)
• Número MDL: MFCD00081656
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: bacterial (Leuconostoc mesenteroides)
• Nivel de calidad: 300
• recombinante: expressed in E. coli
• Formulario: ammonium sulfate suspension
• actividad específica: ≥550 units/mg protein (biuret)
• mol peso: 128 kDa
• condiciones de almacenamiento: (Tightly closed)
• técnicas: cell culture | mammalian: suitable
• Nº de acceso UniProt: UPI0021A4D550
• actividad extraña: creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway.[1] G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).[2]G6PD is a dimer and consists of a single active site at each subunit.[3]

Research area: Cell Signaling

Aplicación

Glucose-6-phosphate dehydrogenase has been used:

• to test ketose reductase activity in developing maize endosperm.[4]
• to determine the levels of mannose in coronary heart disease patient-derived serum[5]
• to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication[6]
• to determine the glucose uptake in cultured human muscle satellite cells[7]

Acciones bioquímicas o fisiológicas

G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.[8]

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Definición de unidad

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Forma física

Suspension in 3.2 M (NH₄)₂SO₄ containing 50 mM Tris and 1 mM MgCl₂, pH 7.5

Otras notas

The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type ABEK (EN14387) respirator filter