G3153

β-Galactosidasa from Escherichia coli

lyophilized powder, ≥500 units/mg protein

• Sinónimos: β-D-galactósido galactohidrolasa, Lactasa
• Número de CAS: 9031-11-2
• Comisión internacional de enzimas: 3.2.1.23 (BRENDA, IUBMB)
• EC Number: 232-864-1
• MDL number: MFCD00130623
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• recombinant: expressed in E. coli
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 465 kDa
• storage temp.: −20°C

Descripción

General description

β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.

Application

β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).

β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.

Biochem/physiol Actions

βLa β-galactosidasa hidroliza la lactosa en sus componentes monosacáridos, glucosa y galactosa. También cataliza la transglucosilación de la glucosa en alolactosa, el inductor de la β-galactosidasa, en un bucle de retroalimentación.

β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.

Packaging

Package size based on protein content

Physical properties

Peso molecular del tetrámero 465 kDa (subunidades de 116,3 kDa cada una)

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Physical form

Stabilized with phosphate buffer and sucrose

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)