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F6057

Sigma-Aldrich

Fluoroshield™ with DAPI

liquid

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About This Item

Código UNSPSC:
12171500
NACRES:
NA.47

Nombre del producto

Fluoroshield™ with DAPI, liquid

descripción

histology mounting medium

Nivel de calidad

200

Formulario

liquid

composición

Sodium Azide, ≤1% (also contains other ingredients)
Tris-HCl

técnicas

immunofluorescence: suitable

intervalo de pH

7.9-8.3

solubilidad

water: soluble

fluorescencia

λex 360 nm; λem 460 nm

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Fluoroshield with DAPI is an aqueous mounting medium fortified with 4′,6-diamidino-2-phenylindole (DAPI), a nuclear counterstain used as a blue-fluorescent DNA probe. It is an anti-fading agent employed for preserving the fluorescence of tissue and cell smears by preventing rapid photobleaching and minimizing the blinking-mediated phenomena.(1) For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Aplicación

Fluoroshield™ with DAPI has been used:
  • as a counterstain in immunocytochemistry to label nuclei of neural progenitor cells
  • as a histology mounting medium for cardiac tissues sections for immunofluorescence studies
  • as mounting solution for immunostained heart sections for nuclei detection

Información legal

FluoroShield is a trademark of ImmunoBioScience Corp.

Producto relacionado

Referencia del producto
Descripción
Precios

03989

Eukitt® Quick-hardening mounting medium, for microscopy

05393

Eukitt® UV, for microscopy

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000427552
0000427552
0000411200
0000411200
0000405985

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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miR-210 suppresses BNIP3 to protect against the apoptosis of neural progenitor cells
Wang F, et al.
Stem Cell Research, 11(1), 657-667 (2013)
S Hamada et al.
Histochemistry, 79(2), 219-226 (1983-01-01)
DNA-DAPI complexes emit strong bluish white fluorescence when excited by ultraviolet light so that even very small amounts of DNA such as those in mitochondria, chloroplasts, and virus particles can be visualized. Moreover, the staining procedure with DAPI is very
Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms
Nascimento DS, et al.
Stem Cell Research & Therapy, 5(1), 5-5 (2014)
Edgar T Hoorntje et al.
PloS one, 13(8), e0203078-e0203078 (2018-08-31)
Likely pathogenic/pathogenic variants in genes encoding desmosomal proteins play an important role in the pathophysiology of arrhythmogenic right ventricular cardiomyopathy (ARVC). However, for a substantial proportion of ARVC patients, the genetic substrate remains unknown. We hypothesized that plectin, a cytolinker
Extracellular matrix-derived extracellular vesicles promote cardiomyocyte growth and electrical activity in engineered cardiac atria
An M, et al.
Biomaterials, 146(1), 49-59 (2017)
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