F2883
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–FITC antibody produced in sheep
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Anti-Mouse IgG FITC
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About This Item
Productos recomendados
origen biológico
sheep
Nivel de calidad
conjugado
FITC conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
Formulario
buffered aqueous solution
técnicas
direct immunofluorescence: 1:128
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
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Descripción general
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody is specific for ) F(ab′)2 fragment of mouse IgG1, G2a, G2b, and G3 subclasses. The antibody preparation is solid phase adsorbed with human serum proteins prior to conjugation to minimize cross reactivity. The antibody preparation is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment is specific for mouse IgG subclasses G1, G2a, G2b, and G3 as demonstrated by Ouchterlony double diffusion using mouse myeloma proteins. The antibody product can be used to avoid background staining due to the presence of Fc receptors.
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment is specific for mouse IgG subclasses G1, G2a, G2b, and G3 as demonstrated by Ouchterlony double diffusion using mouse myeloma proteins. The antibody product can be used to avoid background staining due to the presence of Fc receptors.
Especificidad
Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Inmunógeno
Mouse IgG
Pepsin digest of antiserum
Aplicación
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-FITC antibody may be used in direct immunofluorescence at a minimum antibody dilution of 1:128. The antibody was used at a working dilution of 1:25 to stain microtubules in epidermal cells of Vigna unguiculata or cowpea It was also used for flow cytometry analysis using COS cells and human peripheral blood mononuclear cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Otras notas
Antibody adsorbed with human serum proteins.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Nota de preparación
Adsorbed to reduce background staining with human samples.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Giuliana Napolitano et al.
Mutation research, 749(1-2), 21-27 (2013-08-03)
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D Skalamera et al.
The Plant journal : for cell and molecular biology, 16(2), 191-200 (1998-10-01)
During the infection of cowpea (Vigna unguiculata) by the cowpea rust fungus (Uromyces vignae, race 1 ) the plant nucleus moves towards and away from the invading hypha and eventually moves close to the fungus in the susceptible cultivar while
J R Huth et al.
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