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E6267

Sigma-Aldrich

Earle′s Balanced Salts

With sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

EBSS

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About This Item

MDL number:
MFCD00217343
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 2.2 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

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General description

Earle′s Balanced Salts is a balanced salt solution. It is used as an alternative for conventional simulated body fluids (c-SBF).
Recommended for suspension cultures or whenever cell clumping is a problem.

Application

Earle′s Balanced Salts has been used:
  • to induce autophagy in mouse embryonic fibroblasts
  • in the preparation of melanotroph isolation medium
  • in the preparation of DNAse stock solution

Biochem/physiol Actions

Balanced Salt solution participates in providing cells with water, bulk inorganic ions, and nutrients like carbohydrates essential for normal cell metabolism. It acts as an irrigating, transporting, and diluting fluid to maintain cellular osmotic balance. Balance salt solution helps in maintaining the medium within the physiological pH range (7.2 to 7.6).

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Jinyu Wang et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 73(7), 882-892 (2018-01-19)
Using age of female sexual maturation as a biomarker, we previously identified nuclear receptor interacting protein 1 (Nrip1) as a candidate gene that may regulate aging and longevity. In the current report, we found that the deletion of Nrip1 can
Corrosion performance of MAO coatings on AZ31 Mg alloy in simulated body fluid vs. Earle's Balance Salt Solution
Wilke B M, et al.
Applied Surface Science, 363, 328-337 (2016)
J Bradley Zuchero
Cold Spring Harbor protocols, 2014(8), 826-838 (2014-08-03)
Dorsal root ganglion neurons (DRGs) are sensory neurons that facilitate somatosensation and have been used to study neurite outgrowth, regeneration, and degeneration and PNS and CNS myelination. Studies of DRGs have relied on cell isolation strategies that generally involve extended
Boštjan Rituper et al.
Nature protocols, 8(6), 1169-1183 (2013-05-25)
In order to understand exocytosis and endocytosis, it is necessary to study these processes directly. An elegant way to do this is by measuring plasma membrane capacitance (C(m)), a parameter proportional to cell surface area, the fluctuations of which are
Stephen Beesley et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(45), 28402-28411 (2020-10-28)
The circadian clock is based on a transcriptional feedback loop with an essential time delay before feedback inhibition. Previous work has shown that PERIOD (PER) proteins generate circadian time cues through rhythmic nuclear accumulation of the inhibitor complex and subsequent
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