D9780

Lambda DNA Hind III Digest

for DNA electrophoresis

• Sinónimos: Hind III digested bacteria phage Lambda DNA
• Código UNSPSC: 41141827
• NACRES: NA.25

Propiedades

• grado: for molecular biology
• Nivel de calidad: 200
• Formulario: liquid
• uso: 100 uses (0.1 mg size); 1000 uses (1 mg size); 20 uses (20 μg size); 500 uses (0.5 mg size)
• idoneidad: suitable for electrophoresis (DNA)
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Aplicación

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componentes

Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Otras notas

Contains 8 fragments, 125–23,130 bp

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Nota de preparación

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable