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Key Documents

D8515

Sigma-Aldrich

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA

lyophilized powder

Sinónimos:

DNA, Deoxyribonucleic acid, ds-DNA-Cellulose, DNA-Cellulose

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About This Item

MDL number:
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51

form

lyophilized powder

Quality Level

extent of labeling

3-8 mg double-stranded calf thymus DNA/g

storage temp.

−20°C

General description

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and the linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.

Application

Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.

Quality

May contain a trace of Tris and EDTA.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
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