As per the QC specialist group, in the product CS1090, there isn't any experience with DMSO (all kit's components are without DMSO), so the information cannot provided about DMSO incompatibility. After reviewing the raw data, a good slope was observed with the enzyme; for example, with 6ug (10ul) enzyme, the slope was 0.3-0.5.
CS1090
HMG-CoA Reductase Assay Kit
sufficient for 30 assays (1 mL), sufficient for 100 assays (200 μL)
Sinónimos:
HMGR Assay Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.28
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uso
sufficient for 100 assays (200 μL)
sufficient for 30 assays (1 mL)
Nivel de calidad
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
human ... HMGCR(3156)
Categorías relacionadas
Descripción general
The HMG-CoA Reductase Assay Kit is an important tool for the basic research of cholesterol and other related metabolic pathways.
Aplicación
The HMGR activity kit is designed for the detection of HMG-CoA reductase activity. A major function of the kit is to screen for various inhibitors and activators of the purified catalytic subunit of the enzyme, HMG-CoA reductase, which may play a crucial role in therapeutics. The assay is based on the spectrophotometric measurement of the decrease in absorbance at 340 nm, which represents the oxidation of NADPH by the catalytic subunit of HMGR in the presence of the substrate HMG-CoA.
Acciones bioquímicas o fisiológicas
HMGR (3-hydroxy-3-methylglutaryl-CoA reductase) is a transmembrane glycoprotein located on the endoplasmic reticulum.[1] This enzyme catalyzes the four-electron reduction of HMG-CoA to coenzyme A (CoA) and mevalonate, which is the rate-limiting step in sterol biosynthesis.[2] The activity of HMGR is controlled through synthesis, degradation, and phosphorylation in order to maintain the concentration of mevalonate derived products. In addition to the physiological regulation of HMGR, the human enzyme has been targeted successfully by drugs in the clinical treatment of high serum cholesterol levels.[3][4] Controlling serum cholesterol levels has an important therapeutic role since hypercholesterolemia often leads to the development of atherosclerosis and subsequently to cardiovascular pathologies, which might result in myocardial infarction and stroke. It has been suggested that a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.[5]
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mgSDS
Código de clase de almacenamiento
11 - Combustible Solids
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Artículos
3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.
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Is this suitable for the analysis of compounds dissolved in DMSO for comparing the ability of some compounds to inhibit HMGCR? Specifically, using 1 ul of the compound solution (0.05, 0.5, 5 mM stock buffer) and 1 ul DMSO (solvent alone) as control, the signal @ 340 nM did not significantly decrease after a 10-minute kinetic program (signal capture every 20 seconds according to the protocol). There was only a 15-20 percent decrease in the read. Is this normal, or is DMSO incompatible with the enzyme reaction, or can some improvement be made?
1 answer-
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•What is the concentration of the HMG-CoA (Cat.No. S7447)? and What solvent is it dissolved in?
1 answer-
The concentration of HMG-CoA is the composition of 99.5 % water and 0.5 % (DL-3-Hydroxy-3-methylglutaryl) coenzyme aso-salt trihydrate.
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Product CS1090, kit component of HMG-CoA Reductase Assay Kit Product I5909, Pravastatin, is the concentration 100uM correct?
1 answer-
Yes, the concentration for Pravastatin in this kit is 100 micromolar (100 μM)
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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For Product CS1090, HMG-CoA Reductase Assay Kit, what is the concentration of kit component Product I5909, Pravastatin?
1 answer-
The concentration of Pravastatin, Product I5909 in Product CS1090, HMG-CoA Reductase Assay Kit, is 100 uM solution in assay buffer.
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What is the lowest concentration that you can use Pravastatin and observe inhibition in Product CS1090, HMG-CoA Reductase Assay kit?
1 answer-
For Product CS1090, HMG-CoA Reductase Assay kit we do not observe any inhibition when using Pravastatin at 25 or 50 nM. We observe inhibition of HMG-CoA Reductase with 250 nM pravastatin.
Helpful?
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