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CS0030

Sigma-Aldrich

Senescence Cells Histochemical Staining Kit

sufficient for 100 tests

Sinónimos:

Senescent cells IHC kit, cellular β-galactosidase probe

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.32

usage

sufficient for 100 tests

Quality Level

200

packaging

pkg of 1 kit

storage condition

dry at room temperature

detection method

colorimetric

shipped in

dry ice

storage temp.

−20°C

General description

The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for ß-galactosidase activity at pH 6. ß-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells.
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time.

Application

Senescence Cells Histochemical Staining Kit has been used for:
  • senescence-associated β-galactosidase assay
  • to test for senescence in human pancreatic cancer cells
  • adipose-derived stem cells (ADSCs)
  • cord blood mesenchymal stromal/stem cells (CBMSC)

Biochem/physiol Actions

Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.

Legal Information

Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.

Solo componentes del kit

Referencia del producto
Descripción
  • X-gal solution 4 mL
  • Staining Solution, 10X 15 mL
  • Fixation Buffer 10x 15 mL
  • Reagent B 1.5 mL
  • Reagent C 1.5 mL
  • Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL

related product

Referencia del producto
Descripción
Precios

06693

Timestrip Plus™ -20 °C

94433

β-Galactosidase stain

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

supp_hazards

EUH032

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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B van der Loo et al.
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A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures
G P Dimri et al.
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Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
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